f(A) = (1 - P0) * exp(-k'i * A) + P0
CoLoC-seq Kinetics Model
k'i
P0 represents protected RNA pool proportion; k'i represents digestion rate constant
Extracellular vesicle characterization
Mitochondrial transcriptomics
Organelle RNA localisation
Controlled Level of Contamination coupled to deep sequencing - a novel subcellular transcriptomics approach
CoLoC-seq
Leverages classical enzymatic kinetics to track depletion dynamics of transcripts using RNase gradients
Control experiment with detergent-permeabilized membranes to detect false positives
Mock CoLoC-seq
Distinguishes genuine protection from intrinsic RNase resistance
CoLoC-seq is a novel subcellular transcriptomics approach that uses enzymatic kinetics to distinguish genuine organellar resident RNAs from surface-attached contaminants.
CoLoC-seq probes the global topology of organelle transcriptomes
Nucleic Acids Research
AIDA-based assertion about CoLoC-seq as a novel subcellular transcriptomics approach
Nanopublication: CoLoC-seq method for subcellular RNA localisation